tag:blogger.com,1999:blog-243082782007-04-10T02:51:56.583-07:00siRNA cell transfection reagentshttp://www.blogger.com/profile/16046008342925453008noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-24308278.post-1142701803180389122006-03-18T08:24:00.000-08:002006-10-25T22:28:01.640-07:00Transfection is the introduction of foreign molecules and genetic material such as DNA or RNA into cultured cells. Transfection process typically involves opening transient "holes" in the cellular membrane to allow the entry of extracellular molecules, such as plasmid DNA, protein, small interferring RNA (siRNA), messenger RNA (mRNA), and microRNA(miRNA).<br /><br />Transient transfection refer to introduction of foreign molecules inside the cell without changing it genomic DNA (transient expression). Stable transfection allows incorporation of plasmid DNA into genome of the cell (stable expression).<br /><br />There are various cell transfection methods, including one of the first ones is transfection by calcium phosphate, originally discovered by S. Bacchetti and F. L. Graham in 1977. Other method include electroporation, heat shock, and proprietary transfection reagents from manufacturers such as <a href="http://www.altogen.com" title="Altogen Cell Transfection Reagents">Altogen Biosystems</a>.<br /><br />SiRNA transfection is always a transient event. Maximal effect is typically achieved 48-72 hours after transfection and lasts for 24-96 hours, depending on the efficiency of transfection. Typical transfection protocol includes the procedure of plating the cells, creating transfection complexes by mixing transfection agent with the DNA or RNA, and addition of transfection mixture to the growth medium with the cells.<br /><br />Transfection Links:<br /><br /><a href="http://en.wikipedia.org/wiki/Transfection" title="wiki">WikiPedia: What is transfection?</a><br /><br /><a href="http://www.cell-transfection.com" title="cell transfection">Cell-type specific transfection reagents</a><br /><br />Optimized cell-specific siRNA transfection reagents:<br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/cho.htm"><span style="font-size:85%;">CHO Transfection Reagent (hamster ovary epithelial cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/hepg2.htm"><span style="font-size:85%;">HepG2 Transfection Reagent (human hepatocellular carcinoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/hmec.htm"><span style="font-size:85%;">HMEC Reagent (human mammary epithelial cancer cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mcf-7.htm"><span style="font-size:85%;">MCF-7 Transfection Reagent (human breast cancer cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/ht-29.htm"><span style="font-size:85%;">HT-29 Transfection Reagent (human colorectal adenocarcinoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/htb.htm"><span style="font-size:85%;">HTB Transfection Reagent (human osteosarcoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/huh-7.htm"><span style="font-size:85%;">HuH-7 Transfection Reagent (human hepatoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/huvec.htm"><span style="font-size:85%;">HUVEC Transfection Reagent (umbilical vein endothelial cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mda.htm"><span style="font-size:85%;">MDA-MB Transfection Reagent (breast cancer cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mef.htm"><span style="font-size:85%;">MEF Transfection Reagent (murine embryonic fibroblast cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mes.htm"><span style="font-size:85%;">MES Transfection Reagent (mouse embryonic stem cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mm39.htm"><span style="font-size:85%;">MM39 Transfection Reagent (human tracheal gland cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/mrc-5.htm"><span style="font-size:85%;">MRC-5 Transfection Reagent (human diploid fibroblast cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/nih3t3.htm"><span style="font-size:85%;">NIH3T3 Transfection Reagent (mouse fibroblast cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/nrk.htm"><span style="font-size:85%;">NRK Transfection Reagent (normal rat kidney epithelial cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/shep.htm"><span style="font-size:85%;">SHEP Transfection Reagent (human neuroblastoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/sknas.htm"><span style="font-size:85%;">SKNAS Transfection Reagent (bone marrow neuroblastoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/sknmc.htm"><span style="font-size:85%;">SK-N-MC Transfection Reagent (neuroblastoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/sw-13.htm"><span style="font-size:85%;">SW-13 Transfection Reagent (human adrenocortical carcinoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/u87.htm"><span style="font-size:85%;">U87 Transfection Reagent (glioblastoma cells)</span></a><br /><span style="font-size:85%;"></span><a href="http://www.altogen.com/vero.htm"><span style="font-size:85%;">VERO Transfection Reagent (African green monkey kidney cells)</span></a>siRNA cell transfection reagentshttp://www.blogger.com/profile/16046008342925453008noreply@blogger.com