RNAi Resource: siRNA Transfection

tag:blogger.com,1999:blog-24308278.post-1142701803180389122006-03-18T08:24:00.000-08:002009-04-08T16:03:59.989-07:00RNAi Resource: siRNA TransfectionRNA Interference (<a href="http://www.altogen.com/RNAi.php">RNAi</a>) is utilized in many eukaryotic cells including those found in mammals, which enables protein complex called RISC associate with double-stranded RNA molecules and activate sequence-specific gene silencing (target mRNA degradation). The chain of reactions involved in cells in this manner enables DNA to be transcripted into RNA, which is then translated into proteins. RNAi research is a valuable process in gene expression studies not only for the studies using cell cultures and living organisms (<a href="http://www.altogen.com/invivo_transfection.php">in vivo siRNA</a>), but in synthetic production of the <a href="http://altogen.com/siRNA_transfection.php">siRNA</a> that may eventually be introduced into cells to induce gene suppression as disease-gene-targeted <a href="http://www.altogen.com/RNAi_therapeutics.php">RNAi Therapeutics</a>. RNAi has also been known as post-transcriptional <a href="http://altogen.com/transfection.php">gene silencing</a>, and in 2006, two scientists by the name of Andrew Fire and Craig Mello earned the Nobel Prize in physiology and medicine for their work in RNAi studies. Transfection of siRNA is used in biology laboratories to experimentally induce gene silencing (RNAi) in cultured cells <a href="http://www.cell-transfection.com/products">in vitro</a> and animals <a href="http://www.altogen.com/mirna.php">in vivo</a>. Scientists perform gene expression studies, functional genomics, gene characterization, and discovery of signalling gene pathways by introduction of extracellular molecules, such as small interferring RNA (siRNA), microRNA (miRNA), plasmid DNA expressing shRNA, messenger RNA (mRNA). Transient transfection term refer to introduction of siRNA (or miRNA) molecule inside the cell without affecting its genomic DNA (transient expression), while <a href="http://www.altogen.com/stable_transfection.php">stable transfection</a> happens at incorporation of plasmid DNA into genome of the host cell (stable expression). There are various cell <a href="http://www.altogen.com/transfection_methods.php">transfection methods</a>, including chemical transfection, electroporation, sonoporation, magnetofection, and gene gun injection. Commonly used transfection kits and reagents are nanoparticle-, lipid-, liposome-, and polymer-based. Pre-optimizized transfection kits and products are manufactured by <a title="Altogen Cell Transfection Reagents" href="http://www.altogen.com/">Altogen Biosystems</a>. Optimized siRNA transfection kits include: <a href="http://www.altogen.com/mirna.php">In Vivo Transfection Reagents</a> <a href="http://www.altogen.com/hek-293.php">HEK-293 Transfection Reagent</a> <a href="http://www.altogen.com/mef.php">MEF Transfection Reagent</a> <a href="http://www.altogen.com/hepg2.php">HepG2 Transfection Reagent</a> <a href="http://www.altogen.com/cos-7.php">Cos7 Transfection Reagent</a> <a href="http://www.altogen.com/a549.php">A549 Transfection Reagent</a> <a href="http://www.altogen.com/huvec.php">HUVEC Transfection Reagent</a> <a href="http://www.altogen.com/lncap.php">LNCaP Transfection Reagent</a> <a href="http://www.altogen.com/mcf-7.php">MCF-7 Transfection Reagent</a> Transfection Links: <a title="wiki" href="http://en.wikipedia.org/wiki/Transfection">WikiPedia: What is transfection?</a> <a href="http://www.biologyservices.com/">Biology Research Services</a>: <a href="http://www.altogen.com/support.htm">Stably-Expressing Cell Line Generation</a> <a href="http://www.altogen.com/support.php">RNAi Services</a> <a href="http://altogen.com/mirna.php">In Vivo siRNA Transfection Kits and Reagents</a> Optimized cell-specific siRNA transfection reagents: <a href="http://www.altogen.com/cho.php">hamster ovary epithelial cells</a> <a href="http://www.altogen.com/hepg2.php">human hepatocellular carcinoma cells</a> <a href="http://www.altogen.com/hmec.php">mammary epithelial cancer cells</a> <a href="http://www.altogen.com/mcf-7.php">human breast cancer cells</a> <a href="http://www.altogen.com/ht-29.php">colorectal adenocarcinoma cells</a> <a href="http://www.altogen.com/htb.php">human osteosarcoma cells</a> <a href="http://www.altogen.com/huh-7.php">human hepatoma cells</a> <a href="http://www.altogen.com/huvec.php">umbilical vein endothelial cells</a> <a href="http://www.altogen.com/mda.php">breast cancer cells</a> <a href="http://www.altogen.com/mef.php">murine embryonic fibroblast cells</a> <a href="http://www.altogen.com/mes.php">mouse embryonic stem cells</a> <a href="http://www.altogen.com/mm39.php">tracheal gland cells</a> <a href="http://www.altogen.com/mrc-5.php">human diploid fibroblast cells</a> <a href="http://www.altogen.com/nih3t3.php">mouse fibroblast cells</a> <a href="http://www.altogen.com/nrk.php">normal rat kidney epithelial cells</a> <a href="http://www.altogen.com/shep.php">human neuroblastoma cells</a> <a href="http://www.altogen.com/sknas.php">bone marrow neuroblastoma cells</a> <a href="http://www.altogen.com/sknmc.php">neuroblastoma cells</a> <a href="http://www.altogen.com/sw-13.php">human adrenocortical carcinoma cells</a> <a href="http://www.altogen.com/u87.php">glioblastoma cells</a><div class="blogger-post-footer"><img width='1' height='1' src='https://blogger.googleusercontent.com/tracker/24308278-114270180318038912?l=cell-transfection.blogspot.com'/></div>siRNA cell transfection reagentshttp://www.blogger.com/profile/08731093130899124728noreply@blogger.com1